Three labourers were injured in the collapse.
Public Works Department sources said Executive Engineer R N Mishra and two Assistant Engineers were suspended for alleged negligence on Friday.
The department will wait for the report of a three-member committee formed by the district administration before taking further action. A 30-metre-long slab of the fly-over at Teen Imli Chowk, being built by a private firm, fell on Thursday.
Mishra, the suspended official, had claimed a speeding truck hit the iron rods supporting the slab, which caused the collapse. He denied the allegation that the contractor company was using substandard materials.